offers small- and large-scale preparations of high quality plasmid
DNA for all molecular biology and gene therapy applications. Cytogenomics
offers yields of up to 100 mg. Automated instrumentation is used
to perform rapid plasmid miniprep DNA templates for sequencing
in the 96 well format. A variety of other prep services for BAC,
PAC, cosmid and lambda clones are also offered
uses standard restriction enzyme, PCR, and ligation technology
to clone or subclone known genes. RT-PCR can be applied to clone
cDNAs of interest from supplied RNA, or RNA can be purified by
Cytogenomics. 5’-RACE-PCR can be performed to identify and clone
the 5’ end of a cDNA. 3’RACE can be applied if the 3’ end is unknown.
Genes can be recloned to construct full length clones.
will design appropriate metabolic systems in vector of your choice
to get optimal metabolic functions. Functions are directed by
the client we find appropriate Metabolic solutions in the vector.
We combine pathways to give you a solutions to your problem.
will design your protein for optimal expression in E. coli, yeast,
or baculoviral systems. Proteins can be designed with “HIS-tags”,
“GST-tags”, or other system of your choice. Cytogenomics recommends
a regulatable expression system to provide optimum protein yields,
but will work with the vector system of your selection. Expressed
proteins are analyzed by SDS-PAGE, purified using the affinity
tags or other chromatographic step, and verified by N-terminal
sequence analysis, if requested. Standard proteins are provided
at ~85% purity. Additional purification steps are available at
has applied sophisticated technologies to construct completely
synthetic “designer genes” expressing the exact desired protein.
Genes are synthesized by combining overlapping complementary oligonucleotides,
filling in the gaps with a thermostable nucleotide polymerase,
and amplifying the product by PCR. These amplified products are
cloned into the desired plasmid vector and overexpressed. Genes
up to ~2.0 kbp have been synthesized, but there is no theoretical
limit to the size.
“designer genes” normally have unique DNA sequences that have
been modified to optimize the codon usage for the target host.
In addition, restriction sites, leader sequences, homing signals,
or other modified sequences can be incorporated for special applications.
normally performs site-directed mutagenesis using modifications
of a mega-primer PCR protocol. Using this technology, substitutions
of 1-10 bp can easily be generated. These substitutions are useful
for any site-directed mutagenesis project, and have been often
applied for alanine-scanning type of protein functional probing
or “linker-scanning” analyses. Clones can be verified by sequence
Custom Array development
custom array development for RNA expression studies that are modified
to suit customer needs. We use Incyte clones & where required
from other sources.
- Mini Array
of sizes of upto 200 genes
- Maxi Array
of sizes upto 8000 genes
Array & Tissue specific Arrays